FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14926514
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTAG52100.030.8110776
CTGTAGC63450.025.753287
GTAGCAT62700.025.2576799
TGTAGCA72200.022.333488
TAGTCAT66750.021.7841136
TATAGTC81950.017.756114
ATAGTCA88950.016.590055
TATATAG93650.015.3074842
GATCTAA22100.014.39670445-49
GCCTGTA111450.014.2096185
TAGTTAG24700.013.63930330-34
ACGTTAG25400.013.54638870-74
ATATAGT110650.013.2808873
GATCGGA169000.011.9876761
CGTTAGT29350.011.87043370-74
AGTCATT80900.011.7452897
AGTTAGC31250.010.964787530-34
TTAGCGT32350.010.76935335-39
ACGCCTG153750.010.494973
CGCCTGT153950.010.3408914
Produced by FastQC (version 0.11.5)