FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16509168
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA49500.011.0531049
GCCACTC73250.07.66594088
AGTGAGT44000.07.46605731
GTGAGTA43150.07.3426082
AGCCACT92450.06.151757
AAGCCAC115100.05.56664046
GGCCTAA21700.05.241789375-79
TAACCCC68150.05.17614039
Produced by FastQC (version 0.11.5)