FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14231659
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCTA42050.021.67400720-24
CCTAGCT43000.021.29561220-24
GGTCCTA50000.018.08366835-39
ACCTAGC12700.017.91244520-24
CCCTAGC16350.016.81965620-24
CCTAAGG56250.016.33027640-44
CTAACTA58300.015.78264925-29
TCCTAAG59900.015.23910135-39
CCACTCA49050.015.1168249
GTCCTAA62850.014.63836235-39
GCTAACT67400.013.82267125-29
AGCTAAC69200.013.6489520-24
GCCTAGC14800.013.42514120-24
TAGCTAA70300.013.29201620-24
GCCACTC59350.012.9785288
TAGCGAA72350.012.91527645-49
GTAGCGA73650.012.62866245-49
CTAAGGT76800.012.12937640-44
TCCTAGC19550.011.85715920-24
CCCCTAG6850.011.35010315-19
Produced by FastQC (version 0.11.5)