FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15988862
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAT39550.015.4759179
CTTTAAT124050.012.0147692
TGTAGCA56350.012.0119968
CTGTAGC55850.011.4745557
CCTGTAG56050.010.662746
CTAAGGG56000.010.2859352
CCTTTAA152950.09.8055111
AGTGAGT24300.09.4951111
TTAATTT167850.09.2645724
GATCTAA13250.09.23955945-49
GCTAAGG69450.08.409481
TTTAATT192550.08.1144063
TAAGGGG73400.08.0437463
TAGTCAT54250.07.83102466
ACGTTAG17200.07.784860670-74
AACACTA45500.07.59650479
TATAGTC65900.07.31951574
CGTTAGT18700.07.160406670-74
GTGAGTA33200.07.15678022
TATATAG70450.07.05196142
Produced by FastQC (version 0.11.5)