FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15756161
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGCATGAACGTAATGCTCATAACTTCCCTCTAGACCTAGCTGCTGT2015841.2793979447150863No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1970461.250596512691131Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCATG295350.0116.966734
TATGCAT296100.0116.816683
GTTATGC321600.0108.6411741
TTATGCA328300.0105.798742
TGAACGT361000.094.639079
TGCATGA375400.091.8138965
CATGAAC427350.080.585597
ATGAACG430700.079.6913768
GCATGAA436350.078.809986
AGAGCGT725750.041.6593178
GATCGGA733100.041.2759631
TCGGAAG749650.040.3887673
GAGCGTC768850.039.3333559
AAGAGCG772600.039.0958567
ATCGGAA783400.038.7226452
GGAAGAG861350.035.2763985
GAAGAGC866450.035.0946856
CGGAAGA881200.034.5716174
TCTAGAC254500.027.34535830-34
ACCTAGC250750.027.19225135-39
Produced by FastQC (version 0.11.5)