FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14577723
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCT37800.023.42481820-24
CTAGCTA39300.022.56737520-24
CTAACTA45650.019.36513325-29
CCCTAGC13150.019.05093820-24
GGTCCTA47250.018.64864335-39
CCTAAGG50050.017.72099140-44
GTCCTAA54000.016.5841935-39
GCTAACT54600.016.40176625-29
AGCTAAC55150.016.31651320-24
ACCTAGC14100.015.827250520-24
CTAAGGT57400.015.67759640-44
TCCTAAG57250.015.54213335-39
TAGCTAA63850.014.3413220-24
GCCTAGC15450.013.97834220-24
TCCTAGC16650.013.31678520-24
TAGCGAA84350.011.14692245-49
CCCCTAG6800.011.00998515-19
TAACTAT87050.010.71763925-29
CCAGTGA88550.010.43948350-54
GTAGCGA89650.010.42368545-49
Produced by FastQC (version 0.11.5)