FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15789418
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT62850.016.7232518
AACACTA61400.016.5319947
CACTATA66850.015.399549
GCCCTTA61650.011.2102184
CAAACAC98400.010.68149955
AAACACT101000.09.9788666
TAGCCCT68500.09.8826342
ACCAAAC115050.09.45204453
CCAAACA115950.09.06479554
TATTTAG83900.08.5804719
CCCTTAT101600.06.94395455
TACCAAA166800.06.69223362
ATACCAA200550.06.051571
GTAGCCC119550.05.83573961
CCTTATT139100.05.744746
AGCCCTT130850.05.5588263
TCACTCA62700.05.51326853
CTATAGC49600.05.312176710-14
Produced by FastQC (version 0.11.5)