FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14313536
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA38900.017.9528689
AACACTA39450.017.3375727
ACACTAT41550.016.9811348
AAACACT70300.010.8556586
ACCAAAC77600.09.9330853
CCAAACA82550.09.5119924
CAAACAC86700.08.1372225
TACCAAA127200.06.28627542
ACTATAG29800.05.218297510-14
ATACCAA151600.05.146411
Produced by FastQC (version 0.11.5)