FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19901984
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGG183350.011.9355329
TAGTGTA35500.011.1526756
GTAGTGT43700.010.0481855
TGTGTAG45050.09.1081272
AGTGTAC56550.07.1284967
CTCGTGT61400.06.9170825
GTGTACG128300.06.73282158
TGTAGTG63550.06.34345534
CTGTGTA74400.05.72668361
CTCTGTA79800.05.68306166
TGTCTCG72250.05.67918542
CTGTACG209250.05.5386378
GTGTAGT83800.05.4977783
GTACGCT109500.05.4565133
TCTCGTG91050.05.218164
Produced by FastQC (version 0.11.5)