FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15296081
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACTA46300.024.722227
ACACACT56050.020.421816
ACACTAT64700.018.5814978
AACACAC69800.016.5020285
CACTATA78800.015.8047779
CAACACA88300.013.044854
ACAACAC100900.011.9211893
ATACAAC123300.010.2640011
ATCTAGC25450.09.33820325-29
CTAGCTA25850.09.24942425-29
TCTAGCT33300.07.828913725-29
TACAACA154650.07.8244462
CCGTTAA69950.07.82178264
TATCTAG37050.06.725507325-29
CGTTAAA73150.06.49534655
AGGCCTA44200.06.123996375-79
CCCCTAA41800.05.821167110-114
GGCCCCT45500.05.600948110-114
AGAGCCC48050.05.304088130-134
GTTAAAT97850.05.2236046
Produced by FastQC (version 0.11.5)