FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15113416
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA41750.034.529576
ACGCTAC64400.023.0567997
CGCTACT69050.022.0250938
GCTACTG84650.018.0512759
GTACGCT84950.017.987055
AGGTACG88850.017.0952723
GGTACGC113450.013.9008564
AAGGTAC135750.011.7332882
CTCACTA30250.011.4375646
ACTCACT36800.09.5970065
AAAGGTA221300.07.71364451
TTAACGT55650.06.28618720-24
CTACTGA56800.05.75565210-14
TCACTAT71850.05.71828467
CACTATA62900.05.6149318
GTCCCTA24401.08286495E-55.60550553
TGGGTCG59600.05.579654780-84
GACCCCT61900.05.349979415-19
TAACGTT63900.05.137152725-29
ACCCCTA68750.05.08918815-19
Produced by FastQC (version 0.11.5)