FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15550773
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACAA51350.015.7001854
GCACACA66950.011.9412843
CCCCTTA35000.011.31154254
GTGCACA67050.010.9131761
CCCTTAG43150.010.0091615
TGCACAC81550.09.3618262
CACAAAC93300.08.7178356
ACACAAA95200.08.54414755
TAGAGCC51300.07.9976759
AAACTCA116450.07.47914849
CTTAGAG62300.06.93226347
CAAACTC125350.06.89076388
ACAAACT131850.06.4964897
TTAGAGC71400.06.35115588
CCTTAGA69800.06.29051546
GATCGGA115700.06.1346291
GAGCACA122500.05.6408099
AGAGCAC122250.05.59351978
TCGGAAG125000.05.47384173
GCCCCTT99150.05.23020173
Produced by FastQC (version 0.11.5)