FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14523684
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTACG86750.015.2710111
CGACTTA32100.015.245286
CTTACAC41950.012.1796289
ATTACGA121850.011.3004382
TTACGAA120400.011.1359543
TACGAAA139400.09.921974
GACTTAC53550.08.8699057
GAAATCT165300.08.4027037
ACTTACA61700.08.3976258
ACGAAAT175200.07.393895
AATCTTC190450.07.179279
GTACGTA37500.07.027385125-129
AAATCTT222950.06.48780358
AACGACT95450.06.2641434
CGTACGT44300.06.2412486125-129
ACGTACG49050.06.0764003120-124
ACGACTT101950.05.7884355
CGAAATC254750.05.6217366
GTAACTA47600.05.5958424130-134
TACGTAA54150.05.425074125-129
Produced by FastQC (version 0.11.5)