FastQCFastQC Report
Sat 11 May 2024
EGAF00005185555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17790258
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGCATGAACGTAATGCTCATAACTTCCCTCTAGACCTAGCTGCTGT3230551.8159095837733212No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTAT1242540.6984384374864041TruSeq Adapter, Index 20 (97% over 38bp)
CGCGCATGGTGGATTCACAATCCACTGCCTTGATCCACTTGGCTACATCC284500.1599189848736314No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC256820.1443599075404078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)