FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14749147
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTAT767810.5205792579055589TruSeq Adapter, Index 25 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA379000.034.512769
AGAGCAC403550.032.467028
GATCGGA479550.027.7090031
AAGAGCA487000.027.1548337
TCGGAAG524350.025.3028473
GGAAGAG536900.024.765125
ATCGGAA559600.023.979432
GAAGAGC576900.023.1977466
CGGAAGA605150.022.0553324
CTCGTAT187750.013.66514940-44
TCTCGTA192900.013.382421540-44
AGTGGTC197750.013.30904630-34
CACAGTG201100.013.10881630-34
ACAGTGG217850.012.1603930-34
AATCTCG212350.012.1295540-44
CAGTGGT217700.012.12247330-34
GTCACAG220250.012.04751625-29
AGTCACA225850.011.85716625-29
TCACAGT217650.011.8474330-34
GTGGTCA224800.011.63711335-39
Produced by FastQC (version 0.11.5)