FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14749147
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTTCAGGTGTAGATCT767630.5204572169495633Illumina Single End PCR Primer 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT458000.028.05518
GATCGGA481650.027.2286451
AAGAGCG492600.026.1869037
GAGCGTC503800.025.5335589
TCGGAAG532600.024.5580753
GGAAGAG555300.023.6189865
ATCGGAA574400.022.8791032
GAAGAGC591100.022.0423646
CGGAAGA614700.021.3951784
TAGATCT177250.015.00069640-44
AGATCTC193750.013.51526245-49
TCTCGGT214000.012.87542545-49
GTAGATC217050.012.402595540-44
GGTGGTC220950.012.34014650-54
TGGTCGC221250.012.258347550-54
CGGTGGT239500.011.492558550-54
CTCGGTG240500.011.42080645-49
CAGGTGT235850.011.35903135-39
GTGGTCG243800.011.32528750-54
CGTATCA244300.011.30816860-64
Produced by FastQC (version 0.11.5)