FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16689092
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGA15250.011.235542115-119
GCTAGCG19750.09.258776115-119
GGCTAGC22950.07.8423147115-119
TGGCTAG26750.06.728266115-119
AGGGCCG158050.06.4850211
GGGCCGA170100.06.05456832
TTTCCCC169600.05.81407559
GAGCACA124050.05.686119
AGAGCAC138300.05.10036858
Produced by FastQC (version 0.11.5)