FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14417551
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTTCTAATCTCGTAT144720.10037765775893562TruSeq Adapter, Index 15 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA173050.016.3510539
AGAGCAC180300.015.7334998
GATCGGA199800.014.4457581
TCGGAAG236950.012.1857753
AAGAGCA255250.011.6495087
ATCGGAA258200.011.5732222
GAAGAGC299350.010.1738286
GGAAGAG321850.09.4626585
CTAATCT56150.09.283668535-39
TCTAATC57100.09.25530535-39
CGGAAGA352300.08.9727674
GTTCTAA63800.08.418776535-39
GGGGGGG63100.07.53142262
CACGTGT75950.07.242380630-34
AGTCACG77450.07.21368725-29
TCACGTG77100.06.91023930-34
CTCGTAT82900.06.704859340-44
CAGTCAC94700.06.158174525-29
TTCTAAT95200.06.05039735-39
TCTCGTA99550.05.87275240-44
Produced by FastQC (version 0.11.5)