FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11221463
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACAC33650.017.1173387
ACACACC43450.013.5879958
CCCCTAG8650.013.15127915-19
ACCCAAC46500.012.3869525
CCACACA47950.012.1625276
CACACCT55400.011.30682859
ACCCACA54950.010.8756984
CACCCAA57300.010.3040044
CCCACAC57600.09.9998845
CACCCAC70700.09.168693
CCAACCT67950.09.0065977
CCCAACC59250.08.9922996
CCACCCA63300.08.3062183
CCCTAGA13550.08.28919415-19
CCTAGAC14450.08.27117615-19
GCACCCA77900.08.1392482
AACCTCA81550.07.6811569
AGCACCC83150.07.62534481
TCCACCC81950.07.033642
CTAGACT17700.06.5083720-24
Produced by FastQC (version 0.11.5)