FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16181311
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAATCCATCTCGTAT200340.12380949850107942TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA202500.017.3409141
AGAGCAC216650.016.0161048
GAGCACA219700.015.9248289
TCGGAAG252300.014.268393
ATCGGAA269100.013.5381412
AAGAGCA305450.011.6191757
GAAGAGC311500.011.30106456
GGAAGAG326700.010.9515555
CCTAATC67650.010.91812530-34
CGGAAGA333050.010.8524
CCCTAAT68300.010.708817530-34
CACCCTA73950.09.73487430-34
ACCCTAA78850.09.33077530-34
CTAATCC79000.09.039680535-39
CTCGTAT112200.06.95512540-44
CAGTCAC113500.06.799350725-29
AGTCACC115300.06.79310225-29
CCAGTCA120150.06.554839625-29
TCGTATG122300.06.298336545-49
TCTCGTA127050.06.18751840-44
Produced by FastQC (version 0.11.5)