FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13415020
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC175630.13092041607094138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCC3451.8063729E-67.0946795-99
GGACTAG3753.0516397E-45.7604130-34
GATCGGA118200.05.67214731
GAGCACA113300.05.5278629
CCTAATG21202.2878757E-45.4999821
GCTAGTC3900.00310810845.168514795-99
CTAGCCT4802.853023E-45.099799635-39
GGCTAGT4250.00164067525.08164995-99
Produced by FastQC (version 0.11.5)