FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21360109
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGTG114000.017.9333849
CTAGTAG6650.012.12420530-34
CTGGAGT112000.09.512458
GTACTAG8150.09.00947430-34
AGTGAGT52450.08.45428510-14
GTGAGTA60600.07.5073410-14
ACTAGTA10250.07.304093430-34
TACTAGT10250.07.1636330-34
GCTTAGT20053.1704076E-76.89073131
CTTAGTC23058.8166416E-86.56755072
GAGCTAG8600.06.362591110-114
GCTAGTC8150.06.361076140-144
GAGTGAG74000.06.303545510-14
TTAGTCT28301.7815182E-86.1045443
CTAGTCC8500.06.099149140-144
TGAGTAC78600.05.989586410-14
AGGCTAG9400.05.9734836135-139
TAGGCAC63450.05.78583344
CTGTAGG71500.05.49178841
AGCTAGA10950.05.391612110-114
Produced by FastQC (version 0.11.5)