FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16223398
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCTGATAGTGTAGATCT169270.10433695826238129Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA196750.016.5935081
AGAGCGT201100.016.2590988
GAGCGTC204150.016.1217259
TCGGAAG228600.014.70665553
AAGAGCG226000.014.6589247
ATCGGAA264000.012.4630512
GAAGAGC283650.011.9588526
GGAAGAG330100.010.2530445
TAGTGTA64400.09.74707935-39
CGGAAGA368400.09.3434824
TAGATCT79600.08.1934840-44
ATAGTGT79550.08.16225535-39
AGTGTAG78650.08.05447440-44
TAGGGAA94250.07.30171615-19
GAGTGTT93350.07.017227625-29
GATAGTG97800.07.007158335-39
AGAGTGT99200.06.9952625-29
GTAGGGA98500.06.986667615-19
AGTGTTG107550.06.304911625-29
GTAGATC109050.06.231640-44
Produced by FastQC (version 0.11.5)