FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13086791
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA21150.018.723383
CGACTAA20300.017.0246093
ATACTAA36950.012.6657493
TATACTA36050.012.5871732
TAGAGTA27150.011.938112
CTATACT42050.011.7266071
CTCAGAT52850.09.6046721
GTAGAGT37950.08.4075681
AGAGTAA45300.07.9470093
TACTAAC20852.0008883E-107.9424364
TCGTCTA57850.07.5948542
TACTAAG14357.484048E-67.5261234
ACGACTA55050.07.4577952
CTACTAA27753.274181E-117.00539643
GTTCCTA37450.06.92378572
GTCTAAT21451.7010825E-76.71327834
GACTAAA36903.6379788E-126.2438944
TTCCTAA46550.06.03221563
CACGTAA38153.3469405E-105.6618543
TCACGTA39501.382432E-105.65272052
Produced by FastQC (version 0.11.5)