FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14462151
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCTA38500.029.1711584
CCCTACT42300.026.0399066
CTTAGTC27400.022.5962418
TTAGTCC29650.020.6377839
CCTTAGT30950.020.2370367
TCCCTAC55000.020.157955
CCTACTT71600.015.6855567
TCCTTAG44550.013.8975776
GCTCCCT90650.012.866033
AGTGAGT30100.012.5921341
GTGAGTA33000.011.3648912
TAGCATG28050.010.26633558
TGCTCCC121750.010.1890882
CTACTTT120250.010.0580228
TAAACCC44900.09.4596379
ATGCTCC149200.08.207351
CTACCTC94050.07.6725361
CTCCTTA87450.07.57384355
TACTTTA167800.07.1217219
TACCTCC100650.07.0224422
Produced by FastQC (version 0.11.5)