FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18261415
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTTA50350.019.1601544
CCCTTAG59300.016.6318045
TAGAGCC60800.015.6297559
CTTAGAG91300.011.0390257
TTAGAGC90300.010.84264858
CCTTAGA92250.010.6131926
TGCCCCT99150.010.6060792
CACACAA71350.09.9892974
GCCCCTT108450.09.6257233
GTGCACA77750.09.3166511
GCACACA81300.08.6782163
TGCACAC92050.08.0595012
CTGCCCC176200.06.1043091
CAGCTAG35350.05.90591125-129
CACAAAC151750.05.36072166
GCTAGAC40250.05.1866937130-134
ACACAAA155350.05.09747275
Produced by FastQC (version 0.11.5)