FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12171741
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA14900.018.8517173
CGTCTAA35750.013.0951323
GTTCCTA41750.09.6609972
ACCAATC77250.09.5992316
CCAATCT86050.09.0358827
TACCAAT89650.08.6730365
CACGTAA26450.08.1689863
TCACGTA28900.07.97521832
TTACCAA106950.07.74128344
TAGAGTA45550.07.1156492
ACGACTA45600.07.1078472
GTAGAGT42050.07.0677031
GAGTGGT49500.07.0290471
GTTTTAC122450.06.92607981
ATACTAA66450.06.723
CCCTAAG17251.3660334E-56.67772536
CTATACT62900.06.5687781
TTCCTAA57950.06.46282633
CTCAGAT58600.06.43229771
CGAGCTA27050.06.334698140-144
Produced by FastQC (version 0.11.5)