FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12171741
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA14550.019.298753
CGTCTAA33100.013.7037713
GTTCCTA42500.08.8127512
CACGTAA29500.08.298213
TCACGTA32050.07.19149262
GTCTAAA42500.06.9458034
CCCTAAG16809.233221E-66.85700856
CTCAGAT57300.06.8338881
ACGACTA49050.06.7548532
TCGTCTA73750.06.5434962
ACCAATC72100.06.4908576
AGAGTAA53300.06.48397873
CTATACT64550.06.40335131
GTAGAGT43400.06.3492521
GAGTGGT53050.06.28783561
TTCCTAA57300.06.28265143
TAGAGTA46950.06.13651042
TCGAAGT78050.06.1319661
TACCAAT87300.06.1030235
CGAAGTA76950.05.9905742
Produced by FastQC (version 0.11.5)