FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17316107
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCCA41700.014.6795717
GGGACCC55400.012.7392646
AGTGAGT37150.010.2891171
GTGAGTA43550.09.9189772
GATCGGA174300.08.4346321
TAACCCC47150.08.2477669
ACGGGAC99050.08.2151044
GACCCAC83100.08.1462438
CGGGACC100100.08.0569945
ACCCACC84650.07.6566689
TCGGAAG209450.07.35520123
AGAGCGT217200.06.9297418
CGACATA84250.06.75285967
ATCGGAA238200.06.7401092
CACGGGA126850.06.58307363
AAGAGCG249650.06.2597747
GAGCGTC257000.05.99659739
ATAACCC80850.05.87887768
ACCCTTC85050.05.8771691
GACATAT91850.05.6452598
Produced by FastQC (version 0.11.5)