FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14640932
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTAG46400.024.2035126
TAGTCAT60850.022.3600626
GTAGCAT53400.022.2438559
CTGTAGC55200.020.4753277
TGTAGCA63100.018.3680828
TATAGTC75600.017.9024414
ATAGTCA79500.017.5673545
TATATAG85200.016.906092
ATATAGT98450.014.3381493
GATCTAA19700.012.4991145-49
AGTCATT84250.012.0481257
TAGTTAG20650.011.92312230-34
ACGTTAG21100.011.46399570-74
GCCTGTA117700.010.7036865
CGTTAGT23250.010.28002870-74
ATATATA143650.010.0183951
AGTTAGC27400.09.19605230-34
GTTAGTA28800.08.99889670-74
CGCCTGT143050.08.8572934
GTTAGCG29450.08.3130235-39
Produced by FastQC (version 0.11.5)