FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13302410
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAAG59750.023.3749627
TACACAA60800.022.8528796
GTACACA64100.021.5645355
AAGGTAC86150.016.6360972
AGGTACA87450.016.2242033
GGTACAC103400.013.7911864
CACAAGG108100.013.1864138
AAAGGTA135750.010.8756841
ACAAGGC169800.09.0308599
ACACACT43550.07.273391255-59
CACACTT53000.06.30252555-59
AACGCTA56100.05.85157635-39
ACTCTGT62100.05.100755115-119
ACGCTAT66900.05.057577635-39
TTAACTC68650.05.0335355110-114
Produced by FastQC (version 0.11.5)