FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14536664
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGG119950.010.0809939
AACACTA38300.09.2102687
ACACTAT41300.08.8898638
CACTATA41950.08.2357589
CTCGTGT38000.06.4414295
TGTCTCG43600.06.2750242
GTGTACG78150.05.80345348
GTAGTGT30257.253766E-85.7117975
CTGTACG139350.05.37280858
CTCTGTA61800.05.24216846
Produced by FastQC (version 0.11.5)