FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14011845
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA40050.07.7291217
ACACTAT45800.07.3874588
CACTATA47000.07.35200839
CATGACA88400.06.6667741
TATGACA111550.06.3398591
TATGCAA111500.06.07842351
TAGGCAG54400.05.95842931
TATGACC94650.05.60389331
TATGACG104600.05.4933961
CATGACT55800.05.28084951
CCTAGAT17850.0062484395.24285369
ATGACAC79300.05.1752082
TGTGACA57250.05.14709851
TATTACA120250.05.14602851
Produced by FastQC (version 0.11.5)