FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15675454
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAC13700.043.613829
ACACTAG14150.041.7180527
CACTAGA15050.039.223288
TACACTA36050.017.373236
GTACACT49850.012.708085
CTAGACC12700.09.97598110-14
GGTACAC80700.08.3080654
AAGGTAC85100.08.2207012
AGGTACA92200.08.1319163
TGCTAGC16800.07.7982297135-139
CTAGCTG18100.07.397216135-139
GCTAGCT17300.07.3231926135-139
TTGCTAG21650.06.1177797135-139
Produced by FastQC (version 0.11.5)