FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15850787
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACCAAATCTCGTAT158780.10017168232719298TruSeq Adapter, Index 22 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA226700.011.7178369
AGAGCAC230300.011.5044968
GATCGGA284400.09.5735311
AAGAGCA329450.08.43551357
TCGGAAG345400.07.90038733
GAAGAGC363700.07.68077236
ATCGGAA378400.07.3352622
GGAAGAG394400.07.3019615
CGGAAGA456600.06.22835734
CTCGTAT108550.05.46496740-44
TCTCGTA112800.05.15694440-44
CTAGATC19300.05.073077140-144
Produced by FastQC (version 0.11.5)