FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13805514
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA35000.06.7884847
TATTTAG48650.06.21574749
TAATTAG24803.5426456E-76.1002971
ACACTAT35756.184564E-116.04188068
CACTATA38205.638867E-115.84285649
CCCTTAT60100.05.27114735
GCCCTTA60500.05.2362964
Produced by FastQC (version 0.11.5)