FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13805514
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAA26150.021.7505057
TACGCTA38200.015.4548386
CGCTAAA44600.013.0756758
AGGTACG73800.08.0972453
GCTAAAA83400.07.85577969
GTACGCT84900.07.63218365
AAGGTAC83400.07.3383212
CACTATA35950.07.20970639
ACACTAT35700.06.65517858
AACACTA35407.2759576E-126.3048167
GGTACGC121900.05.37466864
CGCTAAC27400.05.255250550-54
Produced by FastQC (version 0.11.5)