FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11713639
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTC6800.08.47048885-89
GGCTAGT7850.07.887804585-89
AGGCTAG7750.07.24636685-89
GAGCTAG5903.6379788E-127.077852260-64
CTAGATC8500.06.606952140-144
GCTAGCT6654.0199666E-106.0630603140-144
CTAGTCC7659.094947E-126.02345890-94
CGCTAGC8005.456968E-125.9399037140-144
CTAGCTA5157.229446E-75.87175140-144
GATCGGA122500.05.58754251
AGCTAGA8108.6674845E-95.155472360-64
GCTAGAA9101.1023076E-95.063654460-64
CCTAAGA22958.259225E-45.0230861
Produced by FastQC (version 0.11.5)