FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15153682
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTAT538170.3551414105165992TruSeq Adapter, Index 15 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA224650.039.2846269
AGAGCAC237500.037.4319048
GATCGGA278700.032.0009541
TCGGAAG298500.029.916313
ATCGGAA317000.028.4430222
AAGAGCA316050.028.1512037
GGAAGAG373400.024.0202795
GAAGAGC385900.023.316836
CGGAAGA406500.022.1880474
AGTCACA111850.015.78099125-29
CAGTCAC132850.013.67658125-29
GTCACAC129100.013.43818325-29
TCACACG131950.013.017735530-34
CTCGTAT134050.012.68567540-44
CCAGTCA147750.012.37530825-29
TCTCGTA144050.011.91498540-44
CGAATCT144900.011.726068535-39
CTCGAAT159200.011.26973635-39
CACACGT162800.011.12507910-14
ACTCCAG169450.010.90905620-24
Produced by FastQC (version 0.11.5)