FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15153682
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGTGGTGTGTAGATCT579280.3822701307840563Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA284400.031.2356281
AGAGCGT293900.029.8226918
TCGGAAG303600.029.2380983
AAGAGCG318900.027.7118827
ATCGGAA327200.027.2662532
GAGCGTC347600.025.1739719
GGAAGAG388300.023.0176725
GAAGAGC395400.022.4232486
CGGAAGA412900.021.6826294
TAGATCT112250.015.86544140-44
GTGTGTA115900.015.25186435-39
TGTGTAG114550.015.22011240-44
AGTGTAC122000.014.39685625-29
TACAGTG123250.014.204061530-34
ACAGTGG127500.013.9112630-34
GAGTGTA127500.013.79839825-29
AGAGTGT133250.013.267796525-29
GTACAGT137150.012.9744430-34
CAGTGGT135450.012.94595830-34
GGTGTGT142600.012.55766535-39
Produced by FastQC (version 0.11.5)