FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14537623
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTAT788300.5422482066015881TruSeq Adapter, Index 10 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA305000.041.2581679
AGAGCAC317950.039.3966038
GATCGGA330500.038.258491
TCGGAAG367550.034.2199363
ATCGGAA386950.032.671752
AAGAGCA413400.030.6834187
GGAAGAG460900.027.5055925
GAAGAGC465350.027.2735066
CGGAAGA478500.026.3154374
GTTAATC147100.017.26570135-39
CGTTAAT150900.016.6591735-39
GCGTTAA155000.016.20921735-39
TTAATCT165600.014.85867535-39
CTCGTAT170300.014.67686940-44
AGTCACC178200.014.397922525-29
CAGTCAC185700.013.77765725-29
TAATCTC176100.013.60480440-44
TCTCGTA185150.013.57747140-44
CCAGTCA196700.013.01449325-29
AGCGTTA192800.012.94912535-39
Produced by FastQC (version 0.11.5)