FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14537623
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCTGATAGTGTAGATCT816870.5619006628525172Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA338700.036.941081
TCGGAAG363400.034.523663
AGAGCGT376300.033.3463258
ATCGGAA389850.032.292082
AAGAGCG396900.031.651857
GAGCGTC400500.031.2055599
GAAGAGC465450.027.129476
GGAAGAG467850.027.113595
CGGAAGA479600.026.384524
TAGATCT142500.017.7019340-44
TAGTGTA142050.017.66678435-39
AGTGTAG152400.016.26857440-44
ATAGTGT159200.015.926435-39
GTAGATC170650.015.09403640-44
TCATTAA170800.014.75205165-69
AGATCTC187550.013.52664845-49
GAGTGTT187500.013.52284525-29
GATAGTG192100.013.42361635-39
CATTAAA192600.013.41869965-69
ATCATTA195000.013.17233560-64
Produced by FastQC (version 0.11.5)