FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13400613
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGGAAATCTCGTAT175160.13071043839561666TruSeq Adapter, Index 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA127800.023.8273749
AGAGCAC146050.021.0471328
GATCGGA181550.017.2775331
TCGGAAG193050.016.6328143
AAGAGCA212950.014.9082827
ATCGGAA222300.014.5090582
GGAAGAG271450.011.934095
GAAGAGC274200.011.866916
CGGAAGA317850.010.2378894
CAGTCAC76050.08.44375325-29
CTCGTAT74450.08.06441540-44
CCAGTCA86650.07.66006125-29
AGTCACC86200.07.44950625-29
CACACGT92150.07.01533310-14
CACGTCT96650.06.98663810-14
TCTCGTA95350.06.7950640-44
TCGTATG97100.06.50943945-49
ACACGTC107050.06.388578410-14
ACTCCAG112650.06.14771520-24
ACGTCTG110450.06.074633615-19
Produced by FastQC (version 0.11.5)