FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13400613
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGCCTCGGTGTAGATCT181820.13568036029396566Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA179100.017.2584481
AGAGCGT183800.016.6860188
TCGGAAG193600.016.1081093
AAGAGCG212100.014.7990697
ATCGGAA219400.014.4435252
GAGCGTC243100.012.9415139
GAAGAGC271700.011.6587416
GGAAGAG270600.011.4135675
CGGAAGA310000.010.1759394
TAGATCT67550.09.44232940-44
AGAGTGT78900.08.10239825-29
GAGTGTC77250.08.070436525-29
AGTGTCA78400.07.9336925-29
GTAGGGA94000.07.00190415-19
GTGTCAG93350.06.971587725-29
TGTAGGG94650.06.938603415-19
TAGGGAA95850.06.866760315-19
AGATCTC100750.06.6452145-49
AAGAGTG100950.06.60362325-29
GTGTAGA102600.06.55345540-44
Produced by FastQC (version 0.11.5)