FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15679035
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT62550.027.2743878
AACACTA70200.025.0199647
CACTATA93350.018.3525589
AAACACT111100.015.4857296
CGTTAAA53500.014.3972855
CCGTTAA57600.013.8797314
ACAAAAC128900.013.7528543
CAAAACA142200.012.6625444
AAAACAC139550.012.5350785
ATACAAA177450.010.7235731
TCCGTTA81750.010.5780133
GGCCTAA37200.09.40537465-69
TACAAAA211700.08.6802332
GTTAAAT98300.08.0553986
TACACAC50050.07.68074335-39
CTACACA49250.07.483931535-39
CTATAGC50050.07.42129310-14
ACACACT51150.07.374510340-44
GCCCCTA51050.07.2223043100-104
TAGCCAC55600.06.70639210-14
Produced by FastQC (version 0.11.5)