FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12433172
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTC9450.08.52921190-94
CTAGTCC11650.08.15399990-94
GAGCTAG8850.07.969030460-64
AGGCTAG8350.07.411987385-89
GGCTAGT10500.07.265060490-94
AGCTAGA12650.06.9405265-69
TCGCTAG9000.06.236986140-144
GCTAGAA16000.05.75721865-69
CTTGGTT50850.05.66104949
TCGTTAC58850.05.62517175
GTTACCA55850.05.54074247
ATCGTTA59900.05.5265674
Produced by FastQC (version 0.11.5)