FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12708426
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGT105750.050.672792
CCAGTCG145600.037.3519861
AGTCGTA72400.035.6107033
AGTCGTG57250.032.958083
GTCGTAT92650.016.3233764
AGTCGTC74100.013.8008623
GATCGGA131650.011.9754771
GTCGTGT94350.011.2204834
AGAGCGT149950.010.5154498
GAGCGTC174900.09.2623489
TCGGAAG176250.08.8259353
AAGAGCG180700.08.8057087
ATCGGAA199050.08.4671242
GTCGTCT81450.07.51560454
GAAGAGC219350.07.35450276
TCGTGTT74550.06.76217655
TAGATCT51150.06.53022740-44
GGAAGAG266700.06.2647115
TCGTATT126700.06.1956375
GTCGTGA85150.06.08953244
Produced by FastQC (version 0.11.5)