FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15449830
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCT37950.023.6360520-24
CTAGCTA41800.021.59682320-24
GGTCCTA47950.018.55685435-39
CCTAAGG48250.018.41152640-44
GCTAACT52050.017.566925-29
AGCTAAC51500.017.5570320-24
GTCCTAA51450.017.35045435-39
TCCTAAG52700.017.1574835-39
CTAACTA53250.017.00878125-29
CCCTAGC15900.016.661720-24
CTAAGGT56750.015.75533840-44
ACCTAGC16700.014.65652720-24
TAGCTAA63500.014.624619520-24
TCCTAGC17550.013.94666720-24
GCCTAGC13150.012.70079220-24
TAGCGAA74650.012.30519145-49
CCAGTGA76200.012.11201150-54
GTAGCGA79350.011.57633945-49
TAAGGTA85000.010.99329240-44
CAGTGAT87000.010.52587355-59
Produced by FastQC (version 0.11.5)