FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16847492
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAAAGCCACTCAACTCACTATACTATATAGCTCTCTAGCTCTACTAC218280.12956231111431898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA64400.050.073029
GCCACTC83850.038.629918
AGCCACT110850.029.8052317
AAGCCAC118250.027.5748126
CCTAGAC30850.021.237976120-124
ACGTTAA49650.018.7027424
ACAAAGC191400.017.4978373
CGTTAAA54950.016.636845
GGCCTAA42500.015.65208975-79
ATACAAA242400.013.9402521
CTAGCTC49350.013.711699535-39
TACAAAG263950.013.070352
CTAGACC53000.013.014172120-124
TACGTTA72450.012.9234463
AAAGCCA263350.012.84693055
CTCACTA53050.012.83587215-19
ATCCTAG54700.012.399249115-119
CAAAGCC306050.011.5719774
GTTACGT85150.011.0806781
ACTCAAC67200.010.38998310-14
Produced by FastQC (version 0.11.5)