FastQCFastQC Report
Thu 9 May 2024
EGAF00005186310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18555036
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGCATGAACGTAATGCTCATAACTTCCCTCTAGACCTAGCTGCTGT2432541.3109864082182325No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1568590.8453715745957053Illumina Single End PCR Primer 1 (100% over 50bp)
CGCGCATGGTGGATTCACAATCCACTGCCTTGATCCACTTGGCTACATCC265390.14302855569776313No Hit

[OK]Adapter Content

Adapter graph